Document Type : Original Article
Authors
1
Horticulture Department, Faculty of Agriculture, Benha University, Moshtohor, Toukh 13736, Egypt
2
Medicinal and Aromatic Plants Research Department., Horticulture Research Institute. Agricultural Research Center, Egypt.
Abstract
Micronodes were used to propagate Sour orange (Citrus aurantium) through In Vitro technique. Also, two cytokinins i.e. Kinetin, 6-benzyl amino purine at concentrations rate of 0.0, 1.0, 2.0, & 3.0 mg/l were employed. The mutagenesis process used chemical mutagens through culturing of in vitro shootlets on MS medium supplemented with
different concentrations of Sodium azide (NaN3) at rate of 0.04, 0.06, & 0.08%; Colchicine at rate of 0.05, 0.10, & 0.15%. Di methyl sulphanate (DMS( at rate of 0.10, 0.30, & 0.50%; and Di ethyl sulphanate (DES) at rate of 0.10, 0.30, & 0.50%. Also, physical mutagens were subjected to different doses of UV rays ( 2, 4, & 6 hours ); microwave treatments (200 wat) for 10, 20, & 30 seconds; and Gamma rays 50, 75, & 100 Gray. the highest concentration of BAP (3.0 mg/L) is more effective in increasing Shoot numbers. However, the lowest concentration of chemical mutagens i.e. DMS (0.10%) induced the highest Survival% and Shoot length. while, using Sodium azide (0.04, 0.06, & 0.08%) had a harmful effect on Survival% and Shoot length. On the contrary, using Colchicine improved most parameters under study. However, the Vitrification parameter was noticed significantly with all Sodium azide concentrations i.e. 0.08, 0.06, & 0.04% as well as DES 0.30 & 0.50% concentrations as compared with the other treatments. Furthermore, a Molecular marker (ISSR) was done, by Using eight primers revealing that the ratio of polymorphism 81.6% under physical effect was less than compared with chemical mutagenesis 88.4%.
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