Bioactive Defensive Constituents against Lumpy Skin Disease and Levels of Natural Antioxidants in BWgarmon and Its Individual Components

Saleem, Fathy M.M.; Al-Shafei, Mohammed A.; Mubark, Mohammed G.A.; Abd Elnaem, Gamal F.

doi:10.21608/sjas.2024.318847.1457

Bioactive Defensive Constituents against Lumpy Skin Disease and Levels of Natural Antioxidants in BWgarmon and Its Individual Components

Document Type : Original Article

Authors

¹ Agricultural chemistry Dept., Faculty of Agriculture, Minia University, Minia-Egypt

² Chairman of Agricultural Research center (ARC), Professor of Animal Health Research Institute, Giza, Egypt

10.21608/sjas.2024.318847.1457

Abstract

In the present article, five samples were subjected for analysis; the results could be summarized as follows. Five samples were examined for qualitative screening these are as follows: (1)-FPBCS= Fine powdered of black cumin seeds, (2)-PGCP =Peeled garlic clove paste, (3)-FPLP + LJ= Fine powered lemon peels + Lemon Juice, (4)-FPWB= Fine powdered of Wheat bran and (5)- BWgarmon = Paste composed from FPBCS+PGCP+FPLP + LJ+ FPWB were examined for phytochemical
screening which revealed the presence of following secondary metabolites in all samples: steroids, terpenoids, tannins,
coumarins, alkaloids, phenolics, flavonoids and isoprenoids. BWgarmon contain 13 different groups. FPLP + LJ contain the highest concentrations of TPCs (552.0±5.62 mg Gallic acid equivalent GAE) /100g) followed by Fine powdered of Wheat bran (373.7±3.8 mg GAE) /100g) while the lowest concentrations (167.03±1.71) are in FPBCS. The concentrations
of TPCs in the composed paste BWgarmon contain 395±4.1 mg GAE) /100g). TFs (mg Rutin Equivalents /100g) ranged from 69.8±0.75 for FPWB to 135.85 ± 1.68 for FPLP + LJ while BWgarmon reported an averaged value reached to be 115±1.48. The results show that scavenging activities (SA %) of TPCs (mg GAE /100g) assessed by KMnO4 method are higher in FPLP + LJ (297.5±3.2) than those reported in BWgarmon (210.2±2.2). SA (%) of TFs (mg GAE)/100g) ranged from 37.4±0.4 from FPBCS to 72.82±0.7 for FPLP + LJ and the final paste (BWgarmon) reached to be 63.64±0.62. Scavenging activities (%) of total carotenoids (TCs μg/g) ranged from 1.42±0.01 for PGCP to 3.54±0.04 for FPBCS.

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